In Drosophila melanogaster, oocytes develop in egg chambers containing the germline-derived oocyte and nurse cells, and the somatic follicle cells (FCs). Signaling between these cells ensure that the egg chamber forms and patterns correctly. A Toll family receptor, 18-wheeler, which is expressed in the FC layer, was hypothesized to play a role in FC adhesion and in the morphogenesis of the dorsal anterior structures of the eggshell. Dorsal appendage morphogenesis is regulated by the transcription factor, Broad. Due to their complementary expression patterns, Broad and 18-Wheeler might interact. Whole mount immunohistochemistry and confocal microscopy were used to assay Broad protein expression in 18-wheeler mutant FC clones; however no alteration in Broad expression was observed. Intriguingly, altered cell morphology was seen at the boundary between clones expressing two copies of 18-wheeler and those lacking 18-wheeler expression. This is consistent with a role for 18-wheeler in cell adhesion and migration.
|Advisor:||Eldon, Elizabeth D.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 50/04M, Masters Abstracts International|
|Subjects:||Entomology, Cellular biology, Developmental biology|
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