Colonization of vegetation by arbuscular mycorrhizal fungi influences plant growth and nutrient uptake from soil. This study was conducted to examine development of mycorrhizal associations across two pipeline disturbance areas in the State of Wyoming. Both pipeline areas are at fairly high elevation, greater than 2,000 m, and are in semi-arid areas composed principally of sagebrush (Artemisia) steppe vegetation. One is Cooper's Cove where there are three pipelines constructed in different years: 1974, 1999, and 2007. The tendency of AMF infection percentages of the plant samples across these three pipeline areas is increasing as time of recovery increases. Roots from two control sites both have higher infection percentage (39.2%±0.056 and 38.3%±0.047) than all three treatments. Roots from the area disturbed 35 years ago by pipeline installation have the highest infection percentage which is 28.8%±0.072, while 10 years pipeline area is 15.8%±0.054 and 2 years pipeline area is 6.3%±0.034. AM fungal spores density in control sites are 432.87±116.33 and 241.15±15.63 spores/ 25g soil The AM fungal spores density in 35 years pipeline area is 349.67±59.42 spores/ 25g soil, while 10 years pipeline area is 208.60±31.50spores/ 25g soil and 2 years pipeline area is 154.34±14.23 spores/ 25g soil. The PLFA analysis of AMF is 4.85±2.54ug and 5.25±2.44ug fatty acid/g soil in control sites, 3.68±1.89ug fatty acid/g soil in 35 years pipeline area, 4.92±2.01ug fatty acid/g soil in 10 years pipeline area, and 3.89±1.44ug fatty acid/g soil in 2 years pipeline area. The other site in the study is Doty Mountain where there are two pipelines. One was inoculated with commercial arbuscular mycorrhizal fungi inoculum, but the other was not. The AMF infection percentage of roots from the inoculated area was 25.3%±0.099, while area which was not inoculated was 9.3%±0.033. The density of AM fungal spores in soil from the inoculated area was 1436.78±172.53/25g soil, and 350.88±105.85/25g soil in the non-inoculated area. The AMF content of PLFA biomarker is 3.24±1.81ug fatty acid/g soil in inoculated area, while it is 2.06±1.44ug fatty acid/g soil in non-inoculated area.
|Advisor:||Williams, Stephen, Stahl, Peter|
|School:||University of Wyoming|
|School Location:||United States -- Wyoming|
|Source:||MAI 50/03M, Masters Abstracts International|
|Keywords:||Arbuscular mycorrhizal fungi, Chornosequence, Inoculation, Pipeline disturbance|
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