Dissertation/Thesis Abstract

Use of quantitative real-time PCR (qPCR) to investigate a potential correlation between populations of the pathogenic amoeba Naegleria fowleri and the bacterium Escherichia coli in Lake Granbury, Texas
by Painter, Stephanie M., M.S., Tarleton State University, 2011, 56; 1503254
Abstract (Summary)

Previous studies have indicated a correlation between the presence of the pathogenic amoeba Naegleria fowleri and the presence of bacteria, such as the indicator Escherichia coli in environmental surface waters. The main objective of this study was to use qPCR methodologies to quantify populations of N. fowleri and E. coli in lake water samples collected in late summer on Lake Granbury in north-central Texas. No statistically significant relationship was identified between the populations of the target microorganisms, indicating that other bacteria, such as cyanobacteria, may be a more significant food source for N. fowleri in Lake Granbury. A highly statistically significant relationship was identified between E. coli detected via qPCR and quantified via the Colilert® method.

Indexing (document details)
Advisor: Pfau, Russell S.
Commitee: Brady, Jeff A., McFarland, Anne M S, Pierce, James R., Thompson, Carol S.
School: Tarleton State University
Department: Chemistry, Geosciences & Environmental Sciences
School Location: United States -- Texas
Source: MAI 50/03M, Masters Abstracts International
Source Type: DISSERTATION
Subjects: Microbiology, Environmental science
Keywords: Escherichia coli, Naegleria fowleri, Recreational water quality
Publication Number: 1503254
ISBN: 9781267080769
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