Previous studies have indicated a correlation between the presence of the pathogenic amoeba Naegleria fowleri and the presence of bacteria, such as the indicator Escherichia coli in environmental surface waters. The main objective of this study was to use qPCR methodologies to quantify populations of N. fowleri and E. coli in lake water samples collected in late summer on Lake Granbury in north-central Texas. No statistically significant relationship was identified between the populations of the target microorganisms, indicating that other bacteria, such as cyanobacteria, may be a more significant food source for N. fowleri in Lake Granbury. A highly statistically significant relationship was identified between E. coli detected via qPCR and quantified via the Colilert® method.
|Advisor:||Pfau, Russell S.|
|Commitee:||Brady, Jeff A., McFarland, Anne M S, Pierce, James R., Thompson, Carol S.|
|School:||Tarleton State University|
|Department:||Chemistry, Geosciences & Environmental Sciences|
|School Location:||United States -- Texas|
|Source:||MAI 50/03M, Masters Abstracts International|
|Subjects:||Microbiology, Environmental science|
|Keywords:||Escherichia coli, Naegleria fowleri, Recreational water quality|
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