Dissertation/Thesis Abstract

Imaging integrin AvB3 expression during tumor growth
by Lu, Xin, M.S., Purdue University, 2011, 72; 1501872
Abstract (Summary)

Integrin αvβ3 plays a pivotal role in tumor angiogenesis. Over the last decade, many radiolabeled cyclic RGD peptides have been evaluated as αvβ3-targeted radiotracers. Despite significant progress in this area, there is a continuing need for more efficient radiotracers with high tumor uptake and tumor-to-background ratios, which are important for early cancer detection.

This project focuses on the biological evaluation of [99mTc(HYNIC-K(NIC)-3P 4-RGD2)(tricine)] (99mTc-K(NIC)-3P-RGD2) as a novel SPECT radiotracer. Biodistribution and imaging studies were performed on athymic nude mice bearing U87MG, MDA-MB-435, A549 or PC3 tumor xenografts. Immunohistochemistry staining was performed using the xenografted tumor tissues to determine their β3 expression levels. It is found that the radiotracer tumor uptake followed the trend: U87MG > MDA-MB-435 ∼ A549 > PC3. The total β3 expression levels follow the general trend: U87MG > MDA-MB-435 ∼ A549 > PC3. There is a linear relationship between the radiotracer %ID/g tumor uptake and the total β3 expression levels. The radiotracer tumor uptake is contributed by the αvβ 3 expressed on both tumor cells and activated endothelial cells of the tumor neovasculature. 99mTc-K(NIC)-3P-RGD2 is able to monitor the tumor αvβ3 expression in a noninvasive fashion.

Indexing (document details)
Advisor: Liu, Shuang
Commitee: Nie, Huiling, Stantz, Keith M., Weatherman, Kara D.
School: Purdue University
Department: Health Sciences
School Location: United States -- Indiana
Source: MAI 50/03M, Masters Abstracts International
Subjects: Pharmacy sciences, Medical imaging
Publication Number: 1501872
ISBN: 978-1-124-99029-3
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