Dissertation/Thesis Abstract

Role of selected extracellular function sigma factors in carbohydrase gene expression in Saccharophagus degradans 2-40
by Sinnott, Corinn Ann, M.S., University of Maryland, College Park, 2011, 69; 1501246
Abstract (Summary)

This project explores the role of selected ECF sigma factors in regulating carbohydrase gene expression in Saccharophagus degradans 2-40. ECF sigma factors most likely to be involved in carbohydrase regulation were identified by scoring for the presence of high sequence similarity in cellulolytic but not in non-cellulolytic bacterial species. Cellvibrio japonicus, Teredinibacter turnerae, and Hahella chejuensis were determined to be the cellulolytic species most appropriate for evaluating transcriptional regulators for a role in carbohydrase regulation. Two sigma factors were identified in this survey. These sigma factor genes were cloned into a plasmid vector and expressed in S. degradans. Cellulase activity was detected for cell lysates of glucose-grown cultures of a strain expressing the sigma factor corresponding to gene 2407. It was concluded that sigma factor 2407 is involved in the regulation of endoglucanases in the S. degradans cellulolytic system.

Indexing (document details)
Advisor: Hutcheson, Steven
Commitee: McIver, Kevin, Stewart, Richard
School: University of Maryland, College Park
Department: Cell Biology & Molecular Genetics
School Location: United States -- Maryland
Source: MAI 50/02M, Masters Abstracts International
Subjects: Genetics
Publication Number: 1501246
ISBN: 978-1-124-96860-5
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