Myo-inositol is an essential metabolite that plays a role in many important biological functions. In eukaryotic cells, myo-inositol-3-phosphate synthase (MIPS) synthesizes myo -inositol from glucose-6-phosphate and myo-inositol oxygenase (MIOX) catabolizes myo-inositol to glucuronic acid. Myo-inositol metabolism has not been explored in the model organism Drosophila melanogaster. This study demonstrates that Drosophila melanogaster can grow on defined media with myo-inositol as the sole carbon/energy source, which suggests that flies can transport and catabolize a sufficient amount of myo-inositol to support growth and development. Proteomic experiments revealed that flies grown on defined media with various carbon/energy sources alter the expression of mitochondrial proteins and mitochondrial associated proteins. Computational results including the percent identity to experimentally established MIOX proteins, the similarity of protein domain and 3-D structures, and the presence of specific transcriptional regulatory elements suggest that the D. melanogaster gene, CG6910 probably encodes MIOX. In addition, the similarity between the patterns of expression of CG6910 in microarrays and MIOX in Western blots suggests that CG6910 encodes MIOX. The hypomorphic phenotype of a D. melanogaster mutant strain with a P-element inserted into CG6910 further indicates that CG6910 encodes MIOX.
|Advisor:||Klig, Lisa S.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 50/03M, Masters Abstracts International|
|Subjects:||Entomology, Cellular biology, Developmental biology|
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