The Early Light-Inducible Protein (ELIP) is expressed in response to high light intensities. ELIPs are proposed to down-regulate chlorophyll synthesis to decrease light absorption and then be degraded after the plant is returned to low light. In Arabidopsis, the ELIP1 gene (At3g22840) is more sensitive to high light than ELIP2 (At4gl4690). This study aims to identify cis regions of the ELIP1 promoter using site-directed mutagenesis in known light regulatory elements and a novel 12 bp element that is unique to both ELIP1 and ELIP2 promoters. The following elements were analyzed: TCAATA at -117 (CAAT), GTGTGAACT at -134 (GT1-like), CACGTG at -170 (G-box), AGATAG at -201 (GATA), TACGTG at -549 (Upstream G-box) and AGGCCACGCCAT at -665 (12 bp). Promoter activity was measured indirectly by the GUS (β-glucuronidase) assay in transgenic plants (n > 20) expressing the (uidA, GUS) gene under the control of either the non-mutated promoter (984 bp control) or the promoter with mutated cis regions. Significant differences were observed between the 984 bp control line and lines containing mutations in the 12bp region, as well as the G-box- UpG-box (one-way ANOVA with a Dunnett's comparison test using a family error rate = 0.05; F = 5.98, df = 12, p < 0.001). These data suggest that the 12 bp cis region, which contains 1-2 sequences of the over-represented in light-induced promoters 1 (SORLIP1) element, in combination with the G-box and UpG-box, is important for the regulation of the ELIP1 gene under HL conditions.
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 50/03M, Masters Abstracts International|
|Subjects:||Plant biology, Genetics|
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