Dissertation/Thesis Abstract

Chloroplast protein degradation during natural leaf senescence in Arabidopsis thaliana
by Vande Wetering, Scott W., M.S., California State University, Long Beach, 2011, 68; 1504551
Abstract (Summary)

Plants allocate nitrogen to photosynthetic proteins to enhance nutrient capture, and supplement growth. In monocarpic species such as Arabidopsis thaliana, onset of reproductive growth induces leaf senescence. Photosynthetic proteins are degraded, and mobilized nitrogen is transported to growing tissues. The location of senescence-induced proteolysis remains ambiguous. Intact chloroplasts isolated from senescent leaves of Arabidopsis thaliana served as an experimental system to determine if proteolysis occurs in organello . The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (LSU) is degraded during senescence, however LSU remained stable for 24 hours in senescent chloroplasts, and was abundant in chloroplasts isolated from severely senescent tissue. Glutamine Synthetase 2 (GS2) and Rubisco Activase (RCA) were rapidly degraded within 2 hours in senescent chloroplasts, and could not be detected in chloroplasts isolated from severely senescent tissue. These results indicate GS2 and RCA can be degraded in organello during leaf senescence, while LSU is degraded by an alternate mechanism.

Indexing (document details)
Advisor: Brusslan, Judy
School: California State University, Long Beach
School Location: United States -- California
Source: MAI 50/03M, Masters Abstracts International
Subjects: Plant biology, Cellular biology
Publication Number: 1504551
ISBN: 978-1-124-99493-2
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