It has been well documented that many southern California marine environments are contaminated with anthropogenic compounds. Some of these compounds are suspected to disrupt endocrine and physiological functions in organisms, and their presence is often associated with historically released and current wastewater discharges, including from wastewater treatment plants (WWTPs). This study measured endocrine parameters and hepatic contaminant concentrations in the flatfish, English sole (Pleuronectes vetulus), resident at a WWTP outfall location and two far-field reference locations. Hepatic protein expression was measured in the same individuals using 2D-GE coupled with MADLI-TOF/TOF MS and de novo sequencing to identify differentially expressed proteins. Fish residing at the outfall location had higher tissue levels of many organic contaminant compounds, disrupted endocrine systems, and altered protein expression. Contaminants were significantly related to the endocrine disrupted conditions and to expression of a variety of hepatic proteins representing functions including: cell signaling, detoxification, oxidative stress and metabolism.
|Advisor:||Kelley, Kevin M.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 50/03M, Masters Abstracts International|
|Subjects:||Molecular biology, Endocrinology, Environmental Studies, Aquatic sciences|
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