My research focuses on the role of the molecular motor, myosin-X (Myo10), in the cellular protrusions called filopodia. Chapter one provides an up-to-date review of Myo10 in a manuscript that is being prepared for submission to the Journal of Cell Science. Chapter two, my main data chapter, was published in Current Biology and describes a novel population of fast-moving Myo10 in filopodia that we discovered using single-molecule imaging techniques. For this paper, I optimized the imaging system used to detect single Myo10 molecules, performed most of the experiments, and made all of the figures. I also helped develop a software program, Kymotracker, that exploits a technique called kymography to track and take measurements of these single molecules in time-lapse videos. In Chapter three, I describe preliminary experiments investigating the role of Myo10 in filopodial adhesions. In Chapter four, I summarize conclusions drawn from my research and discuss important avenues of future research in the field.
|Advisor:||Cheney, Richard E.|
|Commitee:||Bear, James, Jacobson, Ken, Otey, Carol, Sealock, Robert|
|School:||The University of North Carolina at Chapel Hill|
|Department:||Cell & Molecular Physiology|
|School Location:||United States -- North Carolina|
|Source:||DAI-B 73/01, Dissertation Abstracts International|
|Subjects:||Molecular biology, Cellular biology, Biophysics|
|Keywords:||Filopodia, Intrafilopodial transport, Myo10, Myosin-x, Single-molecule imaging, Tirf|
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