Ustilago maydis is a dimorphic fungus with a yeast-like form and a filamentous form, and both are characterized by extensive nuclear movements. This makes Ustilago a model organism for understanding the link between cell morphogenesis and nuclear migration. Lis1 is a microtubule plus-end tracking protein conserved in eukaryotes and is required for nuclear migration and spindle positioning. In this study, I have characterized the homologue of the human LIS1 gene in Ustilago, and demonstrate, for the first time in fungi, that lis1 is essential for cell viability. Using a conditional null mutation I also show that lis1 is necessary for nuclear migration, cell morphogenesis, septum positioning, cell wall integrity, and microtubule organization. I also generated conditional null mutations for other putative nuclear migration genes, num1 and nudC. In addition, I further characterized the morphologically similar Δtea4 mutant phenotype, confirming that Tea4 is required for normal cell morphogenesis and septum positioning.
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 49/05M, Masters Abstracts International|
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