Dissertation/Thesis Abstract

Developing Innovative Metallomics Approaches to Characterize Trace Biometals
by Chan, Qilin, Ph.D., University of Cincinnati, 2011, 141; 3450616
Abstract (Summary)

Many elements have been claimed as essential for health but their precise roles in life are largely uncharacterized. The increasing interests in the metals/metalloids within biological systems have been driving the fast growth of metallomics, a subject originated to establish the links between metals/metalloids and life. The foundation of metallomics relies on precise determination of biological metal(loid) species. The aim of this dissertation is characterization of such species, primarily metalloproteins, in various biological systems using innovative metallomics approaches. These include several multi-technique methods in which the traditional elemental speciation with HPLC-ICPMS (inductively coupled plasma mass spectrometry) and LC-MS/MS based proteomics were combined to meet the challenges of metalloprotein characterization in animals and plants. Two biologically important elements were intensively studied, zinc (Zn) in murine macrophages and selenium (Se) in plants. The metallomics study in macrophages revealed that Zn played a fundamental role in host defense against Histoplasma capsulatum infection, and a few Zn-binding proteins selectively responded to macrophage activation or the pathogen infection. The Se study elucidated the complete profiles of Se metabolites in kale ( Brassica oleracea) and soybean (Glycine max) and more importantly, it discovered the first Se-containing protein in plants, the Se-containing Bowman-Birk proteinase isoinhibitor D-II [Glycine max] derived from the Se-enriched soybean. These findings not only opened new avenues to study Zn’s precise roles in immunity and Se-containing proteins in plants, but also lead the interactions between metallomics and other research areas, such as immunology and proteomics.

Indexing (document details)
Advisor: Caruso, Joseph
Commitee: Gudmundsdottir, Anna, Limbach, Patrick
School: University of Cincinnati
Department: Chemistry
School Location: United States -- Ohio
Source: DAI-B 72/06, Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Analytical chemistry
Keywords: Kale, Macrophage, Metallomics, Soybean, Speciation, Trace biometals
Publication Number: 3450616
ISBN: 978-1-124-59547-4
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