Many elements have been claimed as essential for health but their precise roles in life are largely uncharacterized. The increasing interests in the metals/metalloids within biological systems have been driving the fast growth of metallomics, a subject originated to establish the links between metals/metalloids and life. The foundation of metallomics relies on precise determination of biological metal(loid) species. The aim of this dissertation is characterization of such species, primarily metalloproteins, in various biological systems using innovative metallomics approaches. These include several multi-technique methods in which the traditional elemental speciation with HPLC-ICPMS (inductively coupled plasma mass spectrometry) and LC-MS/MS based proteomics were combined to meet the challenges of metalloprotein characterization in animals and plants. Two biologically important elements were intensively studied, zinc (Zn) in murine macrophages and selenium (Se) in plants. The metallomics study in macrophages revealed that Zn played a fundamental role in host defense against Histoplasma capsulatum infection, and a few Zn-binding proteins selectively responded to macrophage activation or the pathogen infection. The Se study elucidated the complete profiles of Se metabolites in kale ( Brassica oleracea) and soybean (Glycine max) and more importantly, it discovered the first Se-containing protein in plants, the Se-containing Bowman-Birk proteinase isoinhibitor D-II [Glycine max] derived from the Se-enriched soybean. These findings not only opened new avenues to study Zn’s precise roles in immunity and Se-containing proteins in plants, but also lead the interactions between metallomics and other research areas, such as immunology and proteomics.
|Commitee:||Gudmundsdottir, Anna, Limbach, Patrick|
|School:||University of Cincinnati|
|School Location:||United States -- Ohio|
|Source:||DAI-B 72/06, Dissertation Abstracts International|
|Keywords:||Kale, Macrophage, Metallomics, Soybean, Speciation, Trace biometals|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be