Antioxidants from plants scavenge free radicals and prevent reactive oxygen species from having damaging effects in common ailments such as inflammation, atherosclerosis, and Alzheimer's disease. As part of our ongoing studies of antioxidants from tropical edible fruits, we have studied Garcinia intermedia (Pittier) Hammel [synonym: Rheedia edulis (Seem.) Planch. & Triana], native to Central America. In the fruits the following compounds were identified: guttiferone A, guttiferone E, xanthochymol, fukugetin, volkensiflavone and fukugeside. A new compound was tentatively identified in the fruits of this species. The antioxidant activity of guttiferone A in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was IC50 = 46 μM. The antiproliferative effect of guttiferone A against colon cancer cells (HT-29) was IC50 = 15.8 μM.
A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detection (DAD) was developed and validated to quantify seven major phenolic compounds in eight Garcinia species from different geographic regions: G. mangostana, G. xanthochymus, G. spicata, G. livingstonei, G. intermedia, G. hombroniana, G. kola, and G. aristata. Garcina intermedia and G. mangostana had the highest antioxidant activities.
|Advisor:||Kennelly, Edward J.|
|Commitee:||Basile, Dominick V., Cervantes Cervantes, Miguel, Locke, David C., Nee, Michael, Sankaran, Renuka|
|School:||City University of New York|
|School Location:||United States -- New York|
|Source:||DAI-B 72/05, Dissertation Abstracts International|
|Subjects:||Plant biology, Analytical chemistry, Biochemistry|
|Keywords:||Antioxidant activity, Chemoprevention, Colon cancer, Garcinia, Natural products, Phenolic compounds|
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