Dissertation/Thesis Abstract

Imaging aggregates of mutated superoxide dismutase proteins using an atomic force microscope in a fluid cell environment
by Miller, Michelle, M.S., California State University, Long Beach, 2010, 84; 1486389
Abstract (Summary)

Three types of mutant SOD1 (Apo-AS pH3 1 M guanidine, Apo-AS pH3, and Apo-A4V pH7) were studied using the atomic force microscope and a Veeco fluid cell. The fluid cell set up was tested thoroughly and the imaging was optimized by using a silicon cantilever. Sample 1, Apo-AS pH3 1 M guanidine AFM images, showed very large amorphous aggregates, ranging from about 400 to 10,000 nm in length. Sample 2, Apo-AS pH3 images also contained amorphous aggregates, with the most particles per image ranging from 300 to 1,200 nm in length. Sample 3, Apo-A4V pH7 imaged particles, appear to be mainly amorphous aggregations with the least particles per image and a length ranging from 600 to 1,400 nm.

Indexing (document details)
Advisor: Kwon, Chuhee
School: California State University, Long Beach
School Location: United States -- California
Source: MAI 49/01M, Masters Abstracts International
Subjects: Biochemistry, Physics, Biophysics, Materials science
Publication Number: 1486389
ISBN: 978-1-124-24779-3
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