Paramecium tetraurelia exhibit a brief spontaneous backward swimming behavior that is independent of external stimuli. This spontaneous avoiding response (SAR) occurs at regular intervals which increase and decrease in frequency over time. Oscillation of the SAR frequency repeats every 50 minutes and is temperature compensated. Ciliary reversal is triggered by cell depolarization which activates a ciliary voltage-gated calcium channel and is associated with increased intracellular cGMP. Addition of LiCl perturbs the ultradian rhythm of the SAR frequency and myo-inositol restores this rhythm. Thus guanylate cyclase and the inositol signaling pathway may be involved in generation of the ultradian rhythm. Furthermore, addition of theophylline, was found to shorten the oscillation period (tau) and reduce the frequency of avoiding responses overall (mean value). Addition of 8-bromo cGMP was found to also shorten the tau but had no significant effect on the mean value. RNAi silencing of one of the guanylate cyclase genes resulted in elimination of an organized ultradian rhythm and significant reduction in the mean value. These results indicate that guanylate cyclase has a significant impact on the generation of the P. tetraurelia ultradian rhythm.
|Advisor:||Hinrichsen, Robert D.|
|Commitee:||Knoch, Megan E., Major, Robert J.|
|School:||Indiana University of Pennsylvania|
|School Location:||United States -- Pennsylvania|
|Source:||MAI 49/01M, Masters Abstracts International|
|Subjects:||Molecular biology, Genetics, Cellular biology|
|Keywords:||Guanylate cyclase, Inositol, Paramecium, Theophylline, Ultradian, cGMP|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be