Dissertation/Thesis Abstract

Methionine sulfoxide reductase A (MsrA) and aging in the anoxia-tolerant freshwater turtle (Trachemys scripta)
by Bruce, Lynsey Erin, M.S., Florida Atlantic University, 2010, 45; 1485664
Abstract (Summary)

The enzyme Methionine sulfoxide reductase A (MsrA) repairs oxidized proteins, and may act as a scavenger of reactive oxygen species (ROS), making it a potential therapeutic target for age-related neurodegenerative diseases. The anoxia-tolerant turtle offers a unique model to observe the effects of oxidative stress on a system that maintains neuronal function following anoxia and reoxygenation, and that ages without senescence. MsrA is present in both the mitochondria and cytosol, with protein levels increasing respectively 3- and 4-fold over 4 hours of anoxia, and remaining 2-fold higher than basal upon reoxygenation. MsrA was knocked down in neuronally-enriched cell cultures via RNAi transfection. Propidium iodide staining showed no significant cell death during anoxia, but this increased 7-fold upon reoxygenation, suggesting a role for MsrA in ROS suppression during reperfusion. This is the first report in any system of MsrA transcript and protein levels being regulated by oxygen levels.

Indexing (document details)
Advisor: Milton, Sarah
Commitee:
School: Florida Atlantic University
School Location: United States -- Florida
Source: MAI 49/01M, Masters Abstracts International
Source Type: DISSERTATION
Subjects: Molecular biology, Neurosciences, Aging
Keywords:
Publication Number: 1485664
ISBN: 978-1-124-14898-4
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