Silica-based fluorinated bonded stationary phases have shown enhanced selectivity with altered elution orders for molecules differing in hydrophobicity and hydrophilicity in comparison with C8 and C18 reversed phase columns. Hence, two novel silica hydride-based fluorinated bonded phases have been synthesized using a hydrosilation procedure to exploit fluorine-based unique selectivity for polar basic metabolites. Bonded moieties have been characterized by elemental and spectral analyses. Silica hydride-based aqueous normal phase (ANP) chromatography has retention behavior similar to normal phase chromatography, except for the use of water as a part of the binary solvent (>60% acetonitrile:water). In ANP, a higher percentile of nonpolar mobile phase shows increased retention for acids and bases, and nonpolar solutes can also be retained as in reversed phase chromatography (RPC). The synergistic effects of fluorinated phases’ altered selectivity and aqueous normal phase retentivity have been explored for small polar metabolites using high performance liquid chromatography (HPLC) coupled with several detectors. Hydride-based fluorinated stationary phases showed good stability and remarkable reproducibility in retention time with %RSD<1.
|Advisor:||Pesek, Joseph J.|
|Commitee:||Fleming, Patrick E., Terrill, Roger|
|School:||San Jose State University|
|School Location:||United States -- California|
|Source:||MAI 48/06M, Masters Abstracts International|
|Subjects:||Chemistry, Analytical chemistry, Organic chemistry|
|Keywords:||Amino acids, Aqueous normal phase chromatography, Carbohydrates, Fluorinated stationary phases, Organic acids, Silica hydride-based stationary phases|
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