In order to better understand the cell signaling process, non-invasive methods to measure the integrin microclustering and examine the factors affecting the dynamics of these clusters are needed. This dissertation is focused on the development of non-invasive analytical tools to investigate the role of cytoplasmic proteins and cholesterol on the nanoscale dynamic localization of integrins using a technique called FRET, fluorescence resonance energy transfer. FRET is used to detect the proximity of molecules at nanometer distances (1-10 nm) and can be applied in diverse ways with various microscope configurations. FRET images obtained with such setups can be used to quantify the interactions between biomolecules within cells.
|Advisor:||Smith, Emily A.|
|Commitee:||Houk, Robert S., Lin, Victor S., Narasimhan, Balaji, Schmidt-Rohr, Klaus|
|School:||Iowa State University|
|School Location:||United States -- Iowa|
|Source:||DAI-B 71/06, Dissertation Abstracts International|
|Subjects:||Analytical chemistry, Biochemistry|
|Keywords:||Cholestrol, FRET, Fluorescence, Integrins, Membrane organization, Microclustering|
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