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Dissertation/Thesis Abstract

Development of fluorescence-based methods for non-invasive measurement of integrin microclustering
by Dibya, Deepak, Ph.D., Iowa State University, 2010, 117; 3403162
Abstract (Summary)

In order to better understand the cell signaling process, non-invasive methods to measure the integrin microclustering and examine the factors affecting the dynamics of these clusters are needed. This dissertation is focused on the development of non-invasive analytical tools to investigate the role of cytoplasmic proteins and cholesterol on the nanoscale dynamic localization of integrins using a technique called FRET, fluorescence resonance energy transfer. FRET is used to detect the proximity of molecules at nanometer distances (1-10 nm) and can be applied in diverse ways with various microscope configurations. FRET images obtained with such setups can be used to quantify the interactions between biomolecules within cells.

Indexing (document details)
Advisor: Smith, Emily A.
Commitee: Houk, Robert S., Lin, Victor S., Narasimhan, Balaji, Schmidt-Rohr, Klaus
School: Iowa State University
Department: Chemistry
School Location: United States -- Iowa
Source: DAI-B 71/06, Dissertation Abstracts International
Subjects: Analytical chemistry, Biochemistry
Keywords: Cholestrol, FRET, Fluorescence, Integrins, Membrane organization, Microclustering
Publication Number: 3403162
ISBN: 978-1-109-76371-3
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