The mammalian liver is a primary site for iron processing. Excess iron consumption or iron uptake/storage disorders alter hepatic gene expression. The general patterns of change in the hepatic proteome following acute iron overload, however, are poorly understood. To address this, we injected rats with iron dextran to induce liver iron accumulation. Liver non-heme iron levels increased 30-fold at all post-injection times. Solubilized liver proteins with thiourea-containing buffer were subjected to two-dimensional gel electrophoresis. Digital spot quantitation of gel images and analysis of protein tryptic digests by MALDI-TOF/TOF mass spectrometry identified 38 proteins whose expression changed ≥ 1.5 fold following acute iron overload. Most identified proteins involved in urea cycle, anti-oxidant defense, lipid metabolism, and mitochondrial ATP formation increased during iron overload. Fewer proteins decreased, including specific lipid binding proteins. These results provide a foundation for more detailed analyses on the sequential patterns of changes in liver protein expression during acute iron overload.
|Advisor:||McAbee, Douglas D.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 48/04M, Masters Abstracts International|
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