Dissertation/Thesis Abstract

Scanning electrochemical microscopy of DNA monolayers
by Hammond, William Joseph, M.S., California State University, Long Beach, 2009, 118; 1481587
Abstract (Summary)

Scanning Electrochemical Microscopy (SECM), employing electrocatalytic reduction of ferricyanide mediated by the redox intercalators, Methylene Blue or Nile Blue, affixed at specific locations within DNA films self-assembled on gold electrodes, is used for detection of abasic sites within ds-DNA and for sequence-specific detection of TATA-binding protein.

The inherent differences in catalytic tip currents involving ferricyanide and Methylene Blue are used to distinguish monolayer domains containing native ds-DNA versus ds-DNA containing abasic sites within the micron-level lateral spatial resolution of SECM.

The catalytic cycle involving ferricyanide and Nile Blue covalently affixed at discrete sites within DNA films allows sequence-specific and localized detection of TATA-binding protein, a eukaryotic transcription factor which severely distorts DNA upon binding. These experimental results underline the utility of DNA-modified electrodes as convenient platforms for SECM detection schemes that take advantage of efficient, intercalator-mediated, charge transfer through DNA.

Indexing (document details)
Advisor: Slowinski, Krzysztof
School: California State University, Long Beach
School Location: United States -- California
Source: MAI 48/04M, Masters Abstracts International
Subjects: Analytical chemistry, Biochemistry, Physical chemistry
Publication Number: 1481587
ISBN: 978-1-109-65784-5
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