Dissertation/Thesis Abstract

Production and analysis of GFP fusions to determine subcellular localization of proteases identified by bioinformatic analysis in Arabidopsis thaliana
by Belanger, Eileen, M.S., California State University, Long Beach, 2009, 75; 1481610
Abstract (Summary)

How chloroplast proteins are degraded during senescence is still unknown. I hypothesize that chloroplast localized proteases, which are transcriptionally upregulated during senescence, degrade chloroplast proteins. This study used protease-GFP fusions to determine the subcellular localization of 17 of 20 proteases which were identified throught bioinformatics and literature review. Of the 17 protease-GFP fusions constructed, 9 were analyzed using visible GFP fluorescence under confocal laser scanning microscopy (CLSM), 5 of the constructs expressed GFP at a level only detectable on immunoblots using an anti-GFP antibody, and 3 of the constructs expressed GFP at a level too low to be detected. Of the 9 constructs which were analyzed using CLSM, 4 were localized to the chloroplast. Of these 4, S33Aa (At5g13800) and A1–3 (At5g10770) were localized only to the chloroplast. S59a (At1g59660) was localized to the chloroplasts and nuclei, while FtsH8 (At1g06430) was localized to the chloroplasts and mitochondria.

Indexing (document details)
Advisor: Brusslan, Judy
School: California State University, Long Beach
School Location: United States -- California
Source: MAI 48/04M, Masters Abstracts International
Subjects: Molecular biology, Bioinformatics
Publication Number: 1481610
ISBN: 9781109656466
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