Three microsatellite DNA loci were employed in a sampled population of the viviparous black rockfish, Sebastes melanops, from nearshore temperate rocky reefs off Oregon to characterize the mating system of S. melanops, by assessing the incidence of multiple paternity in brooded females and by estimating the relative paternal contributions of fertilizations in multiply-sired broods. Brooded females (n = 17) and samples of their progeny were assayed to determine their multilocus genotypes, and for creation of progeny array data which was then analyzed with parentage software to determine the number of (reconstructed) sires fertilizing each brood. A separate sample of randomly selected adults were also genotyped to calculate the total expected probability of excluding an unrelated male in the population as being the sire of a brood (given a mother's genotype), and was P = 0.97. Parentage analyses revealed that 66% (11/17) of the broods were fertilized by multiple-sires (frequency of multiple paternity) and therefore polyandrous females, and the mean number of sires per brood was 2.1 (SD = 1.0), ranging from 1 to 4 sires. Five broods that revealed two sires also showed significant unequal contributions of fertilizations between the sires (P < 0.05). Documentation of multiple paternity in S. melanops excludes the possibilities of monogamous and polygynous genetic mating systems, and the weak degree of sexual dimorphism between the sexes favors inference for a polygynandrous genetic mating system (both sexes polygamous) over a polyandrous genetic mating system (female polygamy only).
|Advisor:||Wilson, Raymond, Jr.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 48/04M, Masters Abstracts International|
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