Dissertation/Thesis Abstract

Computational physical modeling and design of protein -DNA interactions
by Ashworth, Justin, Ph.D., University of Washington, 2009, 133; 3394201
Abstract (Summary)

Protein-DNA interactions are fundamental in biology, evolution, disease, and biotechnology. This dissertation describes the use of state-of-the-art molecular modeling algorithms and supercomputing to more fully understand the molecular details of protein-DNA interfaces, anal to redesign protein-DNA recognition in order to create cutting edge reagents for genomic medicine. Here I show that all-atom physical modeling protocols derived from "first principles" and knowledge-based biophysical properties can recapitulate many native properties of DNA-binding proteins and enzymes, and can be used to rationally design the cleavage specificity of a homing endonuclease at multiple and adjacent base pairs.

Indexing (document details)
Advisor: Baker, David
Commitee:
School: University of Washington
School Location: United States -- Washington
Source: DAI-B 71/02, Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Systematic, Biochemistry
Keywords: Gene therapy, Homing endonucleases, Protein-DNA interactions
Publication Number: 3394201
ISBN: 9781109609967
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