Rodents' response to novel objects is based upon complex interactions between intrinsic and extrinsic motivations, many of which decrease the validity of measures operationally defining exploratory activity. Two such threats to validity include the frequency of non-exploratory behaviors exhibited during the retention phase of a standard one-trial object recognition (OR) task, as well as the confounding of novelty preference with typical thigmotaxic activity observed in open field environments. Object motion may be employed to minimize the influence of these factors, thereby increasing the construct validity for novelty preference in the one-trial OR paradigm. Prior to assessing the validity of a motion-based one-trial OR task, it is necessary to determine whether object motion is sensitive to novelty preference. Adolescent Sprague Dawley rats were subjected to either a standard or motion-based one-trial OR task from which measures of raw time exploration and discrimination were derived. Object discrimination was also assessed in male and female rats in order to characterize sex-specificity observed in rodent object recognition. Novel objects were explored significantly more than familiar objects in each task with motion-based OR demonstrating significant increases in exploration of both objects. Experiments 1 and 2 also showed that males explored the novel object significantly more than females. These results suggest that a motion-based one-trial OR task was sensitive to novelty preference in adolescent Sprague Dawley rats and may provide a method for enhancing construct validity in one-trial tests of object recognition.
|Advisor:||Booze, Rosemarie M.|
|Commitee:||Booze, Rosemarie M., Coleman, James R., Mactutus, Charles F.|
|School:||University of South Carolina|
|School Location:||United States -- South Carolina|
|Source:||MAI 48/05M, Masters Abstracts International|
|Keywords:||Adolescence, Novel object recognition task, Novelty, Object recognition, One-trial object recognition, Sexual dimorphism|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be