Dissertation/Thesis Abstract

Biophysical analysis of the zebrafish morphogen Squint
by Lee, Joon S., Ph.D., New York University, 2009, 165; 3368986
Abstract (Summary)

The Nodal signal Squint (Sqt) acts as a morphogen during early zebrafish embryogenesis. Quantitative live imaging of fluorescently tagged Sqt was used to investigate the dynamics of Sqt distribution. Sqt-GFP was localized extracellularlly and formed punctate intracellular accumulations. Sqt-GFP generated from a local source distributed rapidly and formed a concentration gradient following a single exponential profile with a length constant of 52.5 + 20.8 μm. This range of Sqt-GFP distribution was consistent with observed ranges of downstream target gene induction. FRAP (Fluorescence Recovery after Photobleaching) analysis revealed a diffusion coefficient of 2.2 + 0.7 μm2/sec for Sqt-GFP. A degradation rate of 7.5 x 10-5 + 6.2 x 10-6 sec-1 was measured by using Sqt fused to the photoconvertible protein Dendra. Simulations using a model based on diffusion restricted by endocytosis revealed general consistency between length constant, diffusion coefficient and degradation rate. Remarkably, the Sqt-GFP diffusion coefficient is ∼20 fold larger than the diffusion coefficient of Dpp-GFP in the Drosophila wing imaginal disc. These results, in conjunction with studies by others, suggest that different diffusion properties may underlie the spatial and temporal scaling of morphogen distribution in different developmental contexts.

Indexing (document details)
Advisor: Schier, Alexander F.
Commitee: Joyner, Alexandra L., Small, Stephen, Treisman, Jessica
School: New York University
Department: Basic Medical Science
School Location: United States -- New York
Source: DAI-B 70/08, Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Cellular biology
Keywords: Diffusion, Morphogenesis, Morphogens, Zebrafish
Publication Number: 3368986
ISBN: 9781109305425