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Dissertation/Thesis Abstract

Consequences of aryl hydrocarbon receptor activation in dendritic cells
by Bankoti, Jaishree, Ph.D., University of Montana, 2009, 175; 3389406
Abstract (Summary)

TCDD (dioxin) causes immunosuppression via activation of the Aryl hydrocarbon receptor (AhR). Dendritic cells (DCs), the professional antigen-presenting cells in the immune system, are adversely affected by TCDD. However, limited information exists regarding the effects of AhR activation on DCs. We evaluated the consequences of AhR activation by TCDD on both steady-state and inflammatory DCs using in vivo and in vitro approaches, respectively. We hypothesized that AhR activation alters DC homeostasis and differentiation leading to generation of immunosuppression. C57Bl/6 mice gavaged with an immunosuppressive dose of TCDD (15 &mgr;g/kg) displayed decreased frequency and number of splenic CD11chigh DCs on day 7. Moreover, TCDD induced a selective loss of the CD11chighCD8&agr;-33D1+ splenic DCs subset, specialized at activating CD4+ T cells, but not the regulatory CD11chighCD8&agr;+DEC205+ splenic DCs. Additionally, TCDD increased the expression of CD86 and CD54, while decreasing the frequency of CD11a and MHC II on the splenic CD11c high DCs. Although TCDD did not alter the number and frequency of CD11clow splenic DCs, it decreased their MHC II and CD11a expression. The loss of CD11chigh DC was independent of an apoptotic event but involved a CCR7-mediated migratory event. Popliteal and brachial lymph node (PBLNs) CD11c+ cells displayed elevated levels of MHC II and CD40, but not DC loss following TCDD exposure. To examine the effects of TCDD on inflammatory DCs, BMDCs were generated in the presence of GM-CSF and vehicle or TCDD. TCDD decreased CD11c expression but increased MHC II, CD86 and CD25 expression on these BMDCs. These effects were AhR-dependent but not exclusively DRE-mediated. Additionally, TCDD modulated antigen uptake and increased LPS- and CpG-induced IL-6 and TNF-&agr; levels but decreased nitric oxide production by the BMDCs. TCDD downregulated LPS- and CpG-induced p65 levels and induced a trend towards upregulation of RelB levels in BMDCs. Despite the induction of suppressive mediators IDO1, IDO2 and TGFβ3, TCDD-BMDCs failed to suppress T cell activation in vivo or induce the generation of adaptive T-regs in vitro. Collectively, our data suggest that AhR activation disrupts DC homeostasis, modulates DC differentiation, TLR responsiveness and induces a regulatory phenotype, effects that may underlie TCDD-induced immunosuppression.

Indexing (document details)
Advisor: Shepherd, David M.
Commitee: Beall, Howard, Lawrence, Paige B., Parker, Keith, Roberts, Kevan, Wetzel, Scott
School: University of Montana
Department: Biomedical Sciences
School Location: United States -- Montana
Source: DAI-B 71/01, Dissertation Abstracts International
Subjects: Molecular biology, Cellular biology
Keywords: Antigen-presenting cells, Aryl hydrocarbon receptor, Dendritic cells, Dioxin, Immunity, T cells
Publication Number: 3389406
ISBN: 978-1-109-55968-2
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