Escherichia coli is commonly used as the production system for recombinant proteins. However, acetate accumulation in fermentation affects cell growth and protein yield. Recent studies have showed that the small RNA SgrS regulates the major glucose transporter mRNA ptsG in a post-transcriptional manner when the metabolic intermediate glucose-6-phosphate is accumulated intracellularly in E. coli K. Here, comparative analysis of the transcription of SgrS and ptsG is performed between E. coli B and E. coli K cultures in both shake flasks and bioreactor. Both strains expressed SgrS when grown on the non-metabolizable glucose analog α-methyl-glucoside. However, under high glucose conditions, only E. coli B showed significant expression of SgrS. This behavior is unaffected by oxygen supply and pH control. E. coli B produced less acetate on glucose than E. coli K in the bioreactor settings. This provides evidence of a possible connection between SgrS and acetate production in aerobic fermentation of E. coli.
|Advisor:||Wang, Nam Sun|
|Commitee:||Shiloach, Joseph, Sriram, Ganesh, Weigand, William A.|
|School:||University of Maryland, College Park|
|School Location:||United States -- Maryland|
|Source:||MAI 48/04M, Masters Abstracts International|
|Subjects:||Microbiology, Chemical engineering|
|Keywords:||Escherichia coli, Glucose uptake, Post-transcriptional regulation, SgrS-ptsG mechanism, Small RNA, pts|
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