Dissertation/Thesis Abstract

In situ activity of NAC11-7 roseobacters in coastal waters off the Chesapeake Bay based on ftsZ expression
by Yao, Daohong, M.S., University of Maryland, College Park, 2009, 60; 1469470
Abstract (Summary)

Phylogenetic analysis of sequences of the cell division gene ftsZ retrieved from the Atlantic coast revealed an interesting subgroup NAC11-7, which was targeted by a specifically designed and optimized Taqman assay in diel samples collected in situ and in parallel on-deck incubations. Rapid changes of ftsZ gene copies and the patchy distribution of other phylotypes at different time points suggested that different NAC11-7 populations were sampled. Strong correlations between ftsZ expression and gene abundance (r-squared=0.62), and between ftsZ expression and water temperature (r-squared=0.73) for in situ samples suggested non-synchronous growth of NAC11-7 group. Contrastingly, a sharp 9:00 AM peak of ftsZ expression in the on deck incubation experiment suggested synchronous growth. We propose a possible mixed model in which a certain fraction of the population is synchronously dividing, while a background of asynchronously dividing NAC11-7 cells also exist, some of which are expressing ftsZ at any given time.

Indexing (document details)
Advisor: Suzuki, Marcelino T.
Commitee: Crump, Byron, Stoecker, Diane K.
School: University of Maryland, College Park
Department: Marine-Estuarine-Environmental Sciences
School Location: United States -- Maryland
Source: MAI 48/01M, Masters Abstracts International
Source Type: DISSERTATION
Subjects: Microbiology
Keywords:
Publication Number: 1469470
ISBN: 978-1-109-38382-9
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