This study was designed to determine the impact of battlefield-like stress on immune function and to analyze if host genomic responses to dengue, plague and Staphylococcal enterotoxin B (SEB) can still predict such exposures despite stress caused immune suppression. Leucocytes isolated from blood samples of 15 US Army Ranger Training Battalion (RTB) Cadets collected before and after 8½ weeks of training were exposed in vitro to the three pathogens to analyze pathogen- and/or stress-induced regulation of gene expression using human cDNA arrays and Real Time PCR. Gene profiling data of the stressed group showed suppression of transcripts important in microbial pattern recognitions, antigen processing and presentations, inflammation, chemotaxis and adhesion. Stress suppressed immune response genes showed no significant gain of activity even after in-vitro treatment of leucocytes with these pathogens. Despite stress suppressed immune responses, other groups of genes regulated by dengue, plague and SEB were identified. Ontological enrichment associated dengue regulated genes with the host's protein synthesis, plague regulated genes with suppression of DNA repair and transcription activities, and some of the SEB-regulated genes with suppression of transcripts important in hematopoisis. Genes that passed the Bonferroni correction were specific to each pathogen and have the potential to serve as direct markers of exposure.
|Advisor:||Yang, David C. H., Jett, Marti|
|School Location:||United States -- District of Columbia|
|Source:||DAI-B 69/12, Dissertation Abstracts International|
|Subjects:||Molecular biology, Immunology|
|Keywords:||Dengue, Physiological stress, Plague, Staphylococcus enterotoxin B|
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