Microfluidics hold much potential for adapting nucleic acid-based molecular diagnostic techniques onto automated platforms to achieve capabilities and performance far superior to the manual, benchtop methods in use today. Especially for infectious disease diagnostics, there is a drive to create rapid, integrated systems capable of sample-to-answer detection with additional benefits such as lower costs and higher reproducibility. This work focuses on the research, design, and development of centrifugal, microfluidic platforms towards in vitro sample-to-answer applications of nucleic acid-based diagnostics for infectious diseases.
A background on current medical diagnostics is first presented, along with the specific biological analysis steps involved in a typical nucleic acid analysis. Additionally, the advantages of adapting medical diagnostics onto microfluidic platforms are discussed. Next, background information, theory, and history are presented for centrifugal compact-disc (CD)-like technologies, along with a brief overview of the field. Then, novel research on single-function microfluidic platforms for nucleic acid analysis is presented, including a CD-based sample preparation platform, a microfluidic nucleic acid amplification system, and a CD-based platform for DNA detection via microarray. Included in this work is the development of a new type of valve for CD microfluidics, which is modeled analytically and validated experimentally.
The remaining sections present novel research on integration of the single-function platforms into two separate sample-to-answer systems. The first CD-based sample-to-answer platform discussed is focused on single-analyte detection of Anthrax by combination of sample preparation and real-time nucleic acid amplification. The second CD-based sample-to-answer platform presented focuses on multi-analyte detection of a wide panel of respiratory viruses by combining sample preparation, nucleic acid amplification, and DNA microarray detection. In-depth numerical modeling of CD microfluidic behavior is also presented as it pertains to uniform microchamber filling.
In conclusion, future prospects for CD-based sample-to-answer platforms are discussed, along with challenges that remain for the CD platform in general. Finally, a forward-looking vision of a future CD-based sample-to-answer system is presented as motivation for continued work.
|Commitee:||Brody, James, Corn, Robert, Lee, Abraham, Tang, William|
|School:||University of California, Irvine|
|Department:||Biomedical Engineering - Ph.D.|
|School Location:||United States -- California|
|Source:||DAI-B 70/11, Dissertation Abstracts International|
|Keywords:||Centrifugal, Microfludics, Molecular diagnostics, Nucleic acids, Sample-to-answer|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be