The main objective of this work is to produce quantitative fluorescence tomography images using functional a priori information from diffuse optical tomography (DOT) and structural a priori information from another, high spatial resolution anatomical imaging modality. In this thesis, the motivation, mathematical framework, system design and description, simulation studies, phantom studies as well as in vivo studies are presented. A fully integrated tri-modality fluorescence, diffuse optical, and x-ray tomography (FT-DOT-XCT) system for small animal imaging is developed in this work. The key motivation for using XCT and DOT, in addition to FT, is that XCT offers anatomical information while DOT provides optical background heterogeneity to improve FT images further. The quantitative accuracy of this hybrid system was tested with irregular mouse shaped phantom studies and in vivo animal studies. In the final phase of the study, a fiber-based MRI-compatible prototype FT system was also developed. The motivation was, again, to produce quantitative FT images using DOT functional a priori information and MRI anatomical a priori information. The research conducted in this thesis work has potential to benefit basic biologists by providing a system that can quantitatively resolve the fluorescence concentration and lifetime parameters in heterogeneous media. Such a system can be utilized for a broad range of application ranging from monitoring stem cell trafficking and differentiation to diagnosis of cancer. The same approach can also be translated into clinical settings and benefit various patient groups in the future.
|Advisor:||Gulsen, Gultekin, Nalciolgu, Orhan|
|Commitee:||Gulsen, Gultekin, Mandelkern, Mark, Nalciolgu, Orhan|
|School:||University of California, Irvine|
|Department:||Physics - Ph.D.|
|School Location:||United States -- California|
|Source:||DAI-B 70/12, Dissertation Abstracts International|
|Keywords:||Fluorescence tomography, Molecular imaging, Multimodality, Quantitative, X-ray CT|
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