P19 embryonal carcinoma cells differentiate into neuron-like cells in the presence of retinoic acid. We conducted a quantitative proteomic analysis of changes in protein expression accompanying the differentiation process using isobaric tag technology coupled with LC-MS/MS. Replicate quantitative data were obtained for 182 proteins in total cell lysates. This comparison between rapidly dividing undifferentiated cells and neuron-like cells revealed protein changes reflecting withdrawal from the cell cycle accompanied by a dynamic reorganization of the cytoskeleton and an up-regulation of mitochondrial biogenesis. Mitochondria isolated from P19 and neuron-like cells provided replicate quantitative information on the relative abundance of 60 mitochondrial proteins. P160 myb-binding protein, Mybbp1a, was one of the proteins most markedly down-regulated during neuronal differentiation. Mybbp1a may repress the transcription coactivator PGC-1 and thus inhibit transcription of nuclear genes encoding mitochondrial proteins in undifferentiated cells. We confirmed that down-regulation of Mybbp1a is coordinated with the increased expression of PGC-1 during differentiation. The combined effect of these changes may stimulate the enhanced mitochondrial biogenesis that accompanies neuronal differentiation.
|School:||State University of New York at Stony Brook|
|School Location:||United States -- New York|
|Source:||DAI-B 69/01, Dissertation Abstracts International|
|Keywords:||Cytoskeleton, Mitochondria, Neural development, Neuronal differentiation, Proteomics|
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