Dissertation/Thesis Abstract

Quantitative proteomic analysis of the changes accompanying P19 cell neuronal differentiation
by Watkins, Jermel Wayne, Ph.D., State University of New York at Stony Brook, 2007, 150; 3299710
Abstract (Summary)

P19 embryonal carcinoma cells differentiate into neuron-like cells in the presence of retinoic acid. We conducted a quantitative proteomic analysis of changes in protein expression accompanying the differentiation process using isobaric tag technology coupled with LC-MS/MS. Replicate quantitative data were obtained for 182 proteins in total cell lysates. This comparison between rapidly dividing undifferentiated cells and neuron-like cells revealed protein changes reflecting withdrawal from the cell cycle accompanied by a dynamic reorganization of the cytoskeleton and an up-regulation of mitochondrial biogenesis. Mitochondria isolated from P19 and neuron-like cells provided replicate quantitative information on the relative abundance of 60 mitochondrial proteins. P160 myb-binding protein, Mybbp1a, was one of the proteins most markedly down-regulated during neuronal differentiation. Mybbp1a may repress the transcription coactivator PGC-1 and thus inhibit transcription of nuclear genes encoding mitochondrial proteins in undifferentiated cells. We confirmed that down-regulation of Mybbp1a is coordinated with the increased expression of PGC-1 during differentiation. The combined effect of these changes may stimulate the enhanced mitochondrial biogenesis that accompanies neuronal differentiation.

Indexing (document details)
Advisor: Bogenhagen, Daniel
Commitee:
School: State University of New York at Stony Brook
School Location: United States -- New York
Source: DAI-B 69/01, Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Neurology
Keywords: Cytoskeleton, Mitochondria, Neural development, Neuronal differentiation, Proteomics
Publication Number: 3299710
ISBN: 9780549436102
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