Quantitative phase imaging is of interest in fields, such as cell biology and integrated optics, which require measurement of the thickness and refractive properties of weak phase objects embedded in a medium. Existing methods of processing Nomarski differential interference contrast (DIC) images post acquisition can provide high contrast, high resolution quantitative phase images of these types of objects, but require further development to obtain quantitative information.
This dissertation extends the implementation of the spiral phase integration of phase-shifted differential interference contrast images in three main areas: experimentation through evaluation of its sources of error, calibration of the spiral phase integration result, and investigation of novel applications to cell biology. A model of the Senarmont compensator configuration required for accurate phase shifting is derived, evaluated and compared with Nomarski phase-shifting differential interference contrast.
The spiral phase integration method is shown to extract accurate quantitative phase information from experimentally acquired phase-shifted differential interference contrast images of partially absorbing embedded objects. Simulation and experiment show that images obtained using the Sernamont compensator configuration of DIC microscopy are qualitatively similar to those obtained with the traditional Nomarski configuration, but theoretical differences exist between the two systems.
These results both demonstrate and improve the experimental use of the differential interference contrast microscope as a high quality phase measurement tool.
|Advisor:||Cogswell, Carol J., Piestun, Rafael|
|School:||University of Colorado at Boulder|
|School Location:||United States -- Colorado|
|Source:||DAI-B 69/11, Dissertation Abstracts International|
|Subjects:||Biomedical engineering, Electrical engineering|
|Keywords:||Differential interference contrast microscopy, Microscopy, Spiral phase integration|
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