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Dissertation/Thesis Abstract

Identification and characterization of a heme responsive element in the hrg-1 promoter
by Sinclair, Jason, M.S., University of Maryland, College Park, 2008, 102; 1473513
Abstract (Summary)

Despite its biological significance, little is known about how animals sense and respond to heme to maintain homeostasis. C. elegans is a heme auxotroph, which makes it an excellent model to identify and dissect heme homeostasis pathways. Using C. elegans we have identified HRG-1, a vesicular heme transporter that is transcriptionally upregulated when environmental heme is low. The current study seeks to address how hrg-1 is regulated by heme. Here, we show that a putative 23 base pair (bp) heme-responsive element (HRE) and GATA-binding motifs are necessary for heme-dependent regulation of hrg-1. The HRE comprises both enhancer and repressor elements and works in conjunction with ELT-2 to regulate hrg-1 expression. We propose that the HRE could be used as a molecular tool in C. elegans to tightly regulate internal gene expression by modulating environmental heme. Our ultimate goal is to identify the trans-acting factor to eventually create a whole animal sensor for monitoring organismal heme homeostasis.

Indexing (document details)
Advisor: Hamza, Iqbal
School: University of Maryland, College Park
Department: Animal Sciences
School Location: United States -- Maryland
Source: MAI 48/03M, Masters Abstracts International
Subjects: Molecular biology, Cellular biology
Keywords: Heme, Transcription
Publication Number: 1473513
ISBN: 978-1-109-56552-2
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