Many leaders underestimate the importance and value of understanding the influence of humor on organizational culture. Humor's influence on organizational culture is under-researched. This quantitative, correlational study examined the relationship between humor styles and organizational culture within Southwest Ambulance. Expanding the framework of Romero and Cruthirds' (2006) Organizational Humor Model (OHM), the researcher used the Humor Styles Questionnaire (HSQ) (Martin, Puhlik-Doris, Larsen, Gray, & Weir, 2003) to measure humor styles, identified and defined as affiliative, self-enhancing, aggressive, and self-defeating, and the Organizational Description Questionnaire (ODQ) (Bass & Avolio, 1992) to measure organizational culture, identified and defined as transactional and transformational. Analysis using Pearson's Product-Moment Correlation showed no relationship between humor styles and organizational culture within Southwest Ambulance. This unique study contributed to the sparse empirical literature on humor styles and organizational culture and explored humor styles as a dual-purpose tool to influence and assess organizational culture.