In order to be effective dressings for the treatment of infected wounds in the battlefield, it is essential that composite materials made from the biopolymers Cellulose and Chitosan contain broad-spectrum antibiotic drugs. These drugs must first be encapsulated into microcapsules in order to protect them from the manufacturing process, ensuring that they remain in a usable form in the final product. Here we demonstrate the successful encapsulation of Ciprofloxacin HCl into Sporopollenin Exine Capsules (SEC) to produce Cipro@SEC, followed by incorporation of Cipro@SEC into a polymer composite consisting of a mixture of Cellulose and Chitosan dissolved in 1-butylmethylimidazolium chloride, an ionic liquid solvent. We also overcome the challenge that arises from Ciprofloxacin HCl’s solubility in water by developing the use of 2-propanol in the place of water for the gelification of the composites and the removal of the ionic liquid solvent.
Ciprofloxacin HCl solution and SEC were mixed together under a high vacuum in order to encapsulate dry Ciprofloxacin HCl into the inner cavity of the SEC, producing Cipro@SEC. Five sequential encapsulations were carried out to ensure complete filling of the SEC. Cellulose and Chitosan were dissolved in 1-butylmethylimidazolium chloride under vigorous stirring at 120 °C to produce a polymer composite ([CEL+CS]). Cipro@SEC was added to the polymer composite at 90 °C to produce [CEL+CS+Cipro@SEC]. [CEL+CS+Cipro@SEC] composites were cast into Teflon molds on Mylar film and allowed to gelify in a desiccated 2-propanol atmosphere. After washing with 2-propanol to remove the 1-butylmethylimidazolium chloride, the composites were dried at high vacuum.
The produced Cipro@SEC were characterized with confocal fluorescence microscopy, analysis of SEM images, fluorescence spectra, XRD spectra, and FTIR spectra. The produced [CEL+CS+Cipro@SEC] composites were characterized with analysis of SEM images, fluorescence spectra, XRD spectra, FTIR spectra, and visual analysis. The Cipro@SEC were found to be completely filled with Ciprofloxacin HCl and were observed to be covered with crystallized Ciprofloxacin HCl from the encapsulation process. The dried [CEL+CS+Cipro@SEC] composite was found to contain three different species of Ciprofloxacin: Ciprofloxacin Hydrochloride, Ciprofloxacin, and an anhydrous form of Ciprofloxacin Hydrochloride.
|Advisor:||Tran, Chieu D.|
|School Location:||United States -- Wisconsin|
|Source:||MAI 82/8(E), Masters Abstracts International|
|Subjects:||Analytical chemistry, Polymer chemistry, Chemistry|
|Keywords:||Cellulose, Chitosan, Ciprofloxacin, Encapsulation, Ionic liquids, Sporopollenin|
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