The utilization of 3D mouse-derived mammary organoid cultures have proven to provide a novel approach to study both normal mammary gland development over a life cycle while also being able to examine how malignancies initiate and progress. Pregnancy induces major changes to cellular output and epigenome that transforms the epithelium of the mammary gland into “milk-producing machines”. The induced epigenetic changes by pregnancy persist in the post-involution mammary epithelial cells and control gene reactivation in response to subsequent re-exposure to pregnancy hormones. With this, a system that tightly controls exposure to pregnancy hormones, activation of genes, and epigenetic changes would allow for increased knowledge of how such molecular changes occur. This work will describe the characterization of ex vivo cultures to mimic the response of mouse mammary-derived organoid cultures to pregnancy hormones and to understand gene regulation and epigenomic reprogramming during pregnancy hormone re-exposure. These findings allude to this system rendering similar epigenetic modifications that previous reports illustrated in an in vivo environment, therefore this model is suited to more closely observe epigenomic rearrangement and investigate unknown players in pregnancy-induced development and epigenetic memory.
|Advisor:||dos Santos, Camila O.|
|Commitee:||Martin, Benjamin L.|
|School:||State University of New York at Stony Brook|
|Department:||Biochemistry and Cell Biology|
|School Location:||United States -- New York|
|Source:||MAI 82/8(E), Masters Abstracts International|
|Subjects:||Biology, Biochemistry, Genetics, Endocrinology|
|Keywords:||Organoid cultures, Pregnancy hormones , Epigenome output , Transcriptional output , Mammary epithelial cells|
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