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Dissertation/Thesis Abstract

Lew.1WR1 Rats Have Altered Hepatic Metabolism During Their Type 1 Diabetes Susceptibility Window
by Wimalarathne, Madushika, M.S., The University of Alabama in Huntsville, 2020, 94; 27838208
Abstract (Summary)

LEW.1WR1 (1WR1) rats have increased type 1 diabetes (T1D) onset with the administration of polyinosinic-polycytidylic acid (PIC). The objective of this study was to compare the insulitis status and liver gene expression in both control and treated 1WR1 rats during disease induction with a T1D-resistant strain of rat, the LEW/SsNHsd (SsNHsd). Using immunohistochemistry we observed higher T cell infiltration in the 1WR1 treated rats compared to the control strain, the SsNHsd PIC-treated rat. Yet we confirmed that the 1WR1 rats were not undergoing significant insulitis at the time point we selected.

Interestingly the 1WR1 treated rat had significantly increased insulin levels compared to the SsNHsd rats, both groups of the 1WR1 rats also had increased liver FAT10 gene expression compared to the SsNHsd. We observed 1WR1 rats have increased expression of genes related to lipid catabolism and lower expression of glucose metabolism-related genes. In conclusion, 1WR1 rats have reduced insulin sensitivity in the context of increased inflammation due to PIC injection which increases beta-cell sensitivity to eventual T-cell mediated destruction.

Indexing (document details)
Advisor: Love-Rutledge, Sharifa
Commitee: Cruz-vera, Luis, Stallsmith, Bruce
School: The University of Alabama in Huntsville
Department: Biological Sciences
School Location: United States -- Alabama
Source: MAI 82/7(E), Masters Abstracts International
Subjects: Immunology, Biochemistry, Cellular biology, Genetics, Endocrinology, Bioinformatics
Keywords: Beta cell-sensitivity, Glucose metabolism, Insulitis, LEW.1WR1 rats, Lipid metabolism, Type 1 diabetes, Polyinosinic-polycytidylic acid (PIC), Liver gene expression, T-cell mediated destruction, Gene expression, Insulin sensitivity , Inflammation
Publication Number: 27838208
ISBN: 9798569904891
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