Tissue damage can occur due to traumatic injuries, congenital defects, or disease. A popular strategy towards designing biomaterials for tissue repair is through the inclusion of an organized, porous microarchitecture to: mimic the hierarchical structure of the extracellular matrix in functional tissues and provide physical guidance for proliferating cells. This dissertation explores a novel method for patterning hydrogels with porous architecture: magnetic templating. This process involves dispersing magnetic alginate microparticles (MAMs) in a hydrogel precursor solution, aligning the MAMs in a uniform magnetic field, crosslinking the hydrogel around the MAM chains, and degrading the MAMs, leaving behind an anisotropic microarchitecture. The MAMs, which consist of magnetic iron oxide nanoparticles embedded in calcium alginate, are fabricated using a microfluidic flow focusing system for high control over microparticle size and iron oxide loading, parameters that are highly consequential in MAM chain assembly and the resulting microarchitecture of the templated channels. The control enabled by this microfluidic process allows for potential parametric studies on the effects of varying IO loading, MAM volume fraction, and MAM diameter on MAM chain assembly, microchannel formation, and bulk mechanical properties. Finally, the propensity of templated hydrogels for cellular infiltration was evaluated through in vitro culture with rat Schwann cells, C2C12 murine fibroblasts, and rat dermal fibroblasts as models for the applications of peripheral nerve repair, skeletal muscle repair, and wound healing. This work ultimately explores the potential of using microfluidics for MAM production and sheds light on the potential for magnetically templated hydrogels for tissue engineering applications.
|Commitee:||Schmidt, Christine E., Lele, Tanmay, Batich, Christopher D.|
|School:||University of Florida|
|School Location:||United States -- Florida|
|Source:||DAI-B 82/6(E), Dissertation Abstracts International|
|Subjects:||Chemical engineering, Biomedical engineering, Nanoscience, Electromagnetics, Fluid mechanics, Histology|
|Keywords:||Hydrogels, Microparticles, Nanoparticles, Regeneration, Scaffolds, Porous structures, Magnetic templating, Tissue damage , Traumatic injuries, Congenital defects, Magnetic alginate microparticles , Microfluid, Fibroblasts, Hydrogel, Skeletal muscle repair, In vitro|
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