Aeromonas hydrophila ML09-119 is an important fish pathogen that severely affects channel catfish aquaculture. To better understand this strain’s virulence factors, outer membrane vesicles (OMVs) were isolated, and their proteome was assessed. Using transmission electron microscopy and dynamic light scattering, OMVs were shown to be monodispersed particles with an average diameter of 120.33 nm. OMV proteins were identified using mass spectrometry, and analysis of the resulting proteome of 74 proteins revealed that many originated from the cytoplasm, but there was an enrichment of outer membrane, periplasmic, and extracellular proteins compared to the total proteome. The majority of the functional classifications were associated with bacterial metabolism. Of the predicted virulence factors, several had a putative function in adherence, and there were type III secretions system proteins as well as three secreted exotoxins. Overall, our data reveal new insights into A. hydrophila OMVs and their potential roles in physiology and virulence.
|Advisor:||Lawrence, Mark L.|
|Commitee:||Karsi, Attila, Hanson, Larry|
|School:||Mississippi State University|
|School Location:||United States -- Mississippi|
|Source:||MAI 82/6(E), Masters Abstracts International|
|Subjects:||Microbiology, Veterinary services, Molecular biology, Optics, Aquatic sciences, Physiology|
|Keywords:||Aeromonad septicemia, Aeromonas, Channel catfish, Microvesicle, Outer Membrane Vesicles (OMVs), Dynamic light scattering, Proteome|
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