Dissertation/Thesis Abstract

Proteomic Analysis of Outer Membrane Vesicles of Aeromonas Hydrophila ML09-119
by Smink, Jordan Ashley, M.S., Mississippi State University, 2020, 52; 28158042
Abstract (Summary)

Aeromonas hydrophila ML09-119 is an important fish pathogen that severely affects channel catfish aquaculture. To better understand this strain’s virulence factors, outer membrane vesicles (OMVs) were isolated, and their proteome was assessed. Using transmission electron microscopy and dynamic light scattering, OMVs were shown to be monodispersed particles with an average diameter of 120.33 nm. OMV proteins were identified using mass spectrometry, and analysis of the resulting proteome of 74 proteins revealed that many originated from the cytoplasm, but there was an enrichment of outer membrane, periplasmic, and extracellular proteins compared to the total proteome. The majority of the functional classifications were associated with bacterial metabolism. Of the predicted virulence factors, several had a putative function in adherence, and there were type III secretions system proteins as well as three secreted exotoxins. Overall, our data reveal new insights into A. hydrophila OMVs and their potential roles in physiology and virulence.

Indexing (document details)
Advisor: Lawrence, Mark L.
Commitee: Karsi, Attila, Hanson, Larry
School: Mississippi State University
Department: Veterinary Medicine
School Location: United States -- Mississippi
Source: MAI 82/6(E), Masters Abstracts International
Subjects: Microbiology, Veterinary services, Molecular biology, Optics, Aquatic sciences, Physiology
Keywords: Aeromonad septicemia, Aeromonas, Channel catfish, Microvesicle, Outer Membrane Vesicles (OMVs), Dynamic light scattering, Proteome
Publication Number: 28158042
ISBN: 9798698593003
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