In this study hyperglycemia and obesity induced by a high-sucrose diet are examined in Drosophila melanogaster third instar larvae. The hypothesis is that inositol, both dietary and endogenously synthesized, will alleviate the high-sucrose induced hyperglycemic and obese conditions. Buoyancy assays demonstrated that wild-type larvae grown on a high-sucrose diet had an obese-like phenotype, which was alleviated with dietary inositol. The high-sucrose diet also increased the levels of glucose in the hemolymph of wild-type larvae, while inositol supplementation reduced hemolymph glucose levels. This study is the first demonstration in animals that Inos, the inositol synthetic gene, transcript levels are regulated in response to dietary inositol. Up-regulation of larval Inos transcript and corresponding inositol synthetic protein levels resulted in a reduced percentage of obese-like larvae and developmental abnormalities, including lack of proboscises and deformed wings. These studies contribute to the understanding of the role of inositol synthesis in obesity, hyperglycemia, and development.
|Advisor:||Klig, Lisa S.|
|Commitee:||Eldon, Elizabeth D., Brusslan, Judy A.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 82/6(E), Masters Abstracts International|
|Keywords:||Development, Drosophila, Inos, Metabolism|
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