Deep Brain Stimulation (DBS) surgery is an FDA approved treatment for drug resistant Parkinson’s Disease (PD). The therapeutic effectiveness of DBS depends on the accurate placement of electrodes in deep brain nuclei targets like the subthalamic nucleus (STN). Current methods of targeting the STN are either invasive and, consequently, risky or incapable of providing sub-millimeter level resolution. Multiphoton imaging of intrinsic tissue signals like autofluorescence and second harmonic generation (SHG) offers a potential solution to this problem. The objective of this thesis is to evaluate the initial design of a 1.2 mm diameter, 186 mm long probe composed of gradient index (GRIN) lenses and perform initial proof of concept testing in-vitro. The GRIN probe provides a field of view (FOV) of ~175 microns, and the full width half maximum (FWHM) of 200 nanometer beads was calculated to be 0.63 microns and 5.28 microns in the lateral and axial directions respectively. It was successfully used to image EGFP labeled brain tissue. Future studies will optimize the GRIN probe design and experimental setup for imaging autofluorescence and SHG in unlabeled tissue, and evaluate the optical aberrations present in the system.
|Advisor:||Gibson, Emily A., Restrepo, Diego|
|Commitee:||Benninger, Richard K., Ojemann, Steven|
|School:||University of Colorado at Denver|
|School Location:||United States -- Colorado|
|Source:||MAI 82/6(E), Masters Abstracts International|
|Subjects:||Bioengineering, Optics, Neurosciences, Surgery, Public health, Health sciences, Medical imaging, Epidemiology|
|Keywords:||Grin endoscope probe, Neurosurgery, Deep Brain Simulation, FDA-approved treatment, Parkinson's Disease, Brain nuceli, Autofluorescence , Brain tissue|
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