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Dissertation/Thesis Abstract

Chronicles of a Pathogen’s Lifestyle: From a Single Cell to a Population
by Seif, Yara , Ph.D., University of California, San Diego, 2020, 301; 28030560
Abstract (Summary)

Bacterial cells are amongst the simplest forms of life. Nevertheless, explaining a cell’s behavior still represents a challenge that the scientific community is grappling with. In this dissertation, I disentangle multiple layers of interacting dynamic complex systems that govern the bacterial response including: 1) the metabolic network, 2) the genetic background, and 3) the nutritional environment. I start by implementing a traditional methodology for the deep metabolic characterization of a single strain of S. aureus, by reconstructing its metabolic network, and proceed to elucidate how this network governs its metabolic response as a result of a changing nutritional environment. Next, I leverage the deluge in genomic sequence data to propagate knowledge from deeply characterized Gram-negative strains including S. enterica to multiple closely related strains, linking genotypic variations to diverging phenotypes, and diverging phenotypes to bacterial lifestyle. Finally, I demonstrate how information inherent to small genomic deviations can reveal the impact of long term colonization on bacterial evolution.

Indexing (document details)
Advisor: Palsson, Berhnard Ø.
Commitee: Raffatellu, Manuela, Nizet, Victor, McCulloch, Andrew, Mesirov, Jill
School: University of California, San Diego
Department: Bioengineering
School Location: United States -- California
Source: DAI-B 82/3(E), Dissertation Abstracts International
Subjects: Bioinformatics, Genetics, Microbiology
Keywords: Evolution, Metabolism, Prokaryotic pathogens, Systems biology
Publication Number: 28030560
ISBN: 9798678108029
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