One of the major limitations in tissue engineering is the inability to adequately vascularize tissue. Numerous studies are ongoing to evaluate potential strategies to enhance vacularization during treatment of bone treatment and trauma. Our interest is in the field of regenerative endodontics, and we studied strategies to enhance vacularization of dental pulp tissue following endodontic procedures. We sought to exploit the differentiation capacity of human mesenchymal stem cells (HMSCs) and dental pulp stem cells (DPSCs) when exposed to extracellular matrix (ECM) of human aortic endothelial cells (HAECs). Cell-based differentiation of multipotent stem cells into endothelial lineage could potentially be used for in vivo formation of blood vessels. Methods: Three cells lines, namely, HMSC, DPSC, and HAEC, were cultured using optimal cell culture conditions. Endothelial cell specific markers were evaluated using immunofluorescence techniques, to study the matrix influence and secretome influence of HAECs on HMSCs and DPSCs at 24-hours. Protein and mRNA expression levels of endothelial cell markers were also evaluated on differentiated HMSCs and DPSCs at 24-hours. Results: Both HMSCs and DPSCs exposed to endothelial cell matrisome and secretome expressed endothelial cell specific markers at both transcriptional and translational levels. Conclusion: The differentiation of HMSCs and DPSCs into endothelial lineage, under the influence of endothelial extracellular matrix, was clearly evident.
|Commitee:||Eapen, Asha, Kohn, Luci, Jennings, David|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 81/12(E), Masters Abstracts International|
|Keywords:||DPSC, HAEC, HMSC, Matrisome, Secretome, Stem cell differentiation|
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