The effects of the physical work environment on employee behavior have become a popular interest in recent organizational behavior research. As office workspaces evolve to embody new physical layouts and new ways of defining work, more research is needed to support current findings in workspace design literature. Using data obtained from 289 adult, voluntary survey respondents who worked in office workspaces at least 10 hours per week, this study measured the extent to which workspace configuration influenced employee job satisfaction. In order to assess workspace configuration, a questionnaire designed for this study was used. Job satisfaction was assessed using Judge, Bono, and Locke’s (2000) five-item self-report index of job satisfaction. Further, this study measured the extent to which the Big Five traits moderated the relationship between workspace configuration and job satisfaction using John and Srivastava’s (1999) 44-item Big Five trait taxonomy. The results of this study supported both hypotheses. Greater workspace openness was significantly and negatively correlated with job satisfaction (p < .01, one-tailed). Of the Big Five traits, it was discovered that agreeableness moderated the relationship between workspace configuration and job satisfaction. The results of this study reveal that, although working conditions may be the same for groups of employees, each individual responds uniquely to the working conditions within which he or she is operating. More research is needed to further examine the way that different personality traits moderate the relationship between workspace configuration and job satisfaction.