Genetic and environmental perturbations impacting neural crest (NC) development can result in pleiotropic structural and functional birth defects, many of which are associated with pediatric syndromes. As developmental precursors, the NC has the unique capacity to give rise to a diverse array of ectodermal and mesoectodermal cell types, from neurons and glia of the peripheral nervous system to the cartilage and bone of the face. In order to transition from a multipotent progenitor to a specific cell type, NC cells must undergo a series of dynamic morphological and behavioral transformations that gradually unfold over time. However, the NC is rare and transient cell population, and the genetic programming that governs the transition through the intermediate stages of differentiation toward a specific cell fate remain poorly understood. In order to investigate the temporal regulation of this process we established a robust in vitro model system of mammalian cranial and trunk NC cell differentiation. Systematic characterization of directed differentiation along the neural and chondrogenic lineages revealed reproducible, temporal benchmarks indicative of the intermediate stages underlying the progression, many of which mimic those described in vivo. Using this culture system, we explicitly interrogated cranial NC cells to uncover the dynamic gene expression changes that occur as the multipotent progenitors transition over time to become peripheral neurons or cartilage matrix-producing chondrocytes. Our analysis revealed that cranial NC cell diversification toward either cell type is mediated through gradual coordination of both common and lineage-specific programming, as well as concurrent suppression of competing cell fates. We further identified distinct transcriptional signatures corresponding to the intermediate state, as well as putative regulators that govern the overall progression in a stage-specific and time-sensitive manner. These data serve as a powerful tool for discovering previously unappreciated mechanisms contributing to cell fate acquisition in the NC, and novel molecular targets of genetic and environmental factors that contribute to NC-related birth defects and disorders.
|Advisor:||Udvadia, Ava J|
|Commitee:||Gutzman, Jennifer H, Oliver, Julie A, Steeber, Douglas A, Svoboda, Kurt R, Blackmore, Murray G|
|School:||The University of Wisconsin - Milwaukee|
|School Location:||United States -- Wisconsin|
|Source:||DAI 81/11(E), Dissertation Abstracts International|
|Subjects:||Developmental biology, Cellular biology, Molecular biology|
|Keywords:||Cell fate determination, Chondrogenesis, Cultured primary neural crest cells, Intermediate progenitor, Peripheral neurogenesis, Temporal gene expression patterning|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be