Dissertation/Thesis Abstract

The Aquaculture and Biology of 'Opihi 'Alinalina (Cellana sandwicensis)
by Mau, Anthony, Ph.D., University of Hawai'i at Manoa, 2019, 225; 27671990
Abstract (Summary)

The present paradigm in global seafood production suggests that the market demands farm-raised seafood over wild-caught seafood. Whether this is due to a shift in market preference or simply an overall increase in farm production, there is an overwhelming amount of empirical evidence for diminishing return of wild stocks. For this reason, development of high-value mollusc species remains a top priority for the aquaculture industry – the ultimate goal being to improve food-security and reduce harvesting pressure on wild fisheries.

For selection of new aquaculture species, it’s critical to understand the life-history to effectively engineer infrastructure, establish technical protocols, and optimize nutrition for production. In Hawai’i, ‘opihi ‘alinalina or yellowfoot limpet (Cellana sandwicensis), a prized mollusc shellfish and significant biocultural resource, has been a prospect aquaculture species for many years; and recently, there has been progress made towards closing the life cycle of C. sandwicensis in captivity.

In this dissertation, the primary research objectives were to i) develop a suitable formulated feed by examining the effect of protein to energy ratio on growth performance of adult yellowfoot limpets in aquaculture; ii) establish suitable methodologies for controlled spawning and larval rearing to settlement; iii) examine the effects of a novel synthetic GnRH-like peptide hormone on limpet reproduction utilizing proteomics and solid-phase-peptide-synthesis; and iv) define the growth of yellowfoot limpets utilizing sclerochonology and stable isotope analysis by secondary ion mass spectrometry.

The current advances of aquaculture technology and biology of C. sandwicensis includes: the fabrication and implementation of state-of-the-art recirculating aquaculture systems for broodstock, settlement, and grow-out; testing of a suitable formulated feed that meets nutritional requirements of the species; development of natural and hormone-controlled spawning techniques using a novel limpet GnRH-like peptide; establishment of methodology for larval rearing to settlement; and the first discoveries of key life-history traits.

This research collective closes the present gap-in-knowledge pertaining to C. sandwicensis in aquaculture, and its biology – development, growth, and reproduction. Future research and development will focus on the settlement of limpets and the underlying mechanisms influencing survival in aquaculture, as well as to support ‘opihi fisheries management through understanding the life-history of C. sandwicensis.

Indexing (document details)
Advisor: Bingham, Jon-Paul
Commitee: Jha, Rajesh, Lee, Cheng-Sheng, Mishra, Birendra, Ho, Kacie
School: University of Hawai'i at Manoa
Department: Molecular Biosciences and Bioengineering
School Location: United States -- Hawaii
Source: DAI-B 81/8(E), Dissertation Abstracts International
Subjects: Aquatic sciences, Animal sciences, Biochemistry
Keywords: Ecology, Life-history, Nutrition, Ontogeny, Reproduction, Settlement
Publication Number: 27671990
ISBN: 9781658401647
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