Although CRISPR/Cas9 has been said to be a 'reasonably specific' method of gene editing, which performs double strand breaks in DNA to remove or add nucleotides, there have been many cases of large scale deletions of nucleotides that were not targeted and 'complex genomic rearrangements' (Kosicki 2018). Given these unwanted off-target deletions that occur under CRISPR/Cas9 gene editing, a statistical method of analysis was performed to predict, given the sgRNA target specific sequence, whether the CRISPR/Cas9 negative selection screening would be successful and result in the removal of one or two nucleotides from the target strand resulting in a frameshift and removing a single protein or isoform. The final findings of the logistic model are surprisingly definitive. First, we notice that the thymine and guanine nucleotides make the most difference in determining the success of the Cas9 screening. Second, with even greater importance, upon looking at the 5’–17th nucleotide position in the sgRNA sequence, which is the specific locus of Cas9 mediated cleavage, we discover that there is a much greater chance of success if the sgRNA nucleotide is a guanine, and a much greater chance of failure if it is a thymine. Similarly, a guanine in the 5’–19th position will lead to a much greater chance of successful cleavage.
|School:||Stevens Institute of Technology|
|School Location:||United States -- New Jersey|
|Source:||MAI 81/7(E), Masters Abstracts International|
|Subjects:||Genetics, Artificial intelligence, Biostatistics|
|Keywords:||Cas9, CRISPR, Frameshift, Genetics, Prediction, sgRNA|
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