My goal is to investigate Lipoxygenase (LOX) activity in siliques as they develop to produce seeds, and yellow with age in ltp4 and ft/ltp4 regenerating Arabidopsis thaliana. Our previous research shows that LTP3/4 and FT are upregulated during the floral transition. Knockdowns of these genes results in their redevelopment with basal rosettes formed from the axillary meristems, an increased lifespan of 2+ years and ft/ltp4 flower 7 months late. There was also an increase in 13-lipoxygenase (13-LOX) activity which catalyzes chloroplast lipid peroxidation after the first round of reproduction and apparent death. Bioinformatic analysis shows that there is a relatively high amount of LTP3/4 inside of the seeds which are located within the siliques. LTP’s are known to shuttle oxylipins in and out of the cell for purposes such as growth development and immune response. We hypothesize, that the mutational loss of LTP3/4 and FT/LTP4 that has resulted in the extended lifespan of these plants during the floral transition, may prevent the rapid movement of a LOX derived oxylipin “death” hormone to the auxiliary meristems at apparent death to initiate programed cell death. Thus, resulting in the growth of the redeveloped Arabidopsis thaliana on the bolts. However, the peroxidation activity of LOX in mutant plants is unknown. Since LTP3/4 expression is relatively high in seeds, I will measure peroxidation in LTP4 and FT/LTP4 siliques utilizing the FOX assay. This study will be helpful in developing techniques to improve on this oxylipin pathway and increase crop yield efficacy.
|Commitee:||Hayes, Michael, Wen, Xin, McCurdy, Allison|
|School:||California State University, Los Angeles|
|Department:||Chemistry and Biochemistry|
|School Location:||United States -- California|
|Source:||MAI 81/7(E), Masters Abstracts International|
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