The purpose of the current study was to investigate the relationships between identified faculty learning styles and the retention of content knowledge as measured by online learning activity choice. A total of 17,589 full time faculty were sent an invitation to participate with 165 choosing to be a part of the study. Using a quasi-experimental quantitative research methodology, faculty completed the Cohen, Oxford, and Chi (2006) Learning Styles Survey in an online module. After completing the survey, faculty chose an instructional object that was customized to one of three learning styles: visual, auditory, or kinesthetic. Faculty completed course content in the instructional object and then completed a comprehension quiz. χ² tests of independence confirmed demographic variables are independent of learning style. An ANOVA test supported the assumption that there is not a stronger learning style for online learning, but that all groups showed the same level of propensity for knowledge retention. A Pearson r correlation analysis supported the idea that completion time is not indicative of a better score on assessments. Finally, independent sample t-tests indicated that there was not a significant difference in matched learning style or unmatched learning style groups for either completion time or comprehension quiz score. The final results of the current study indicated that an online model of faculty development appears to be effective in delivering content to faculty regardless of learning style.